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In addition, metabolic dysfunctions, such as insulin resistance, dyslipidemia, and cardiovascular disease are all associated with hepatic steatosis, and seem to be more related to hepatic fat accumulation and NAFLD than obesity status per se [2, 9, 10]

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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development

There are several risk factors that may contribute to the likelihood of developing benign prostatic hyperplasia (BPH), such as: Age